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The structures of The brand new compounds have been determined on The premise of their spectral data, together with 1D and 2nd NMR, HR-MS and also the gene cluster accountable for the biosynthesis of phenazines was discovered. All phenazine derivatives showed antimicrobial action towards the Gram-favourable Bacillus subtilis, even though compounds one–3 and five also inhibited progress of the Gram-detrimental Escherichia coli.
It truly is nonetheless unclear which genes are responsible for the glycosylation from the endophenazine aglycones, as no this sort of genes have been observed within just or in shut proximity of your epa gene cluster (Desk 4). Therefore, the genes answerable for the transfer and methylation with the rhamnose moiety to both the carboxyl or even the hydroxyl group from the phenazines needs to be Situated elsewhere around the chromosome. Study to determine the genes and enzymes accountable for the glycosylation of the endaphenazines is currently in development.
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